详细介绍
QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
纯化得到至多10 mg的无内毒素高转染级纯质粒或柯斯质粒DNA
- 纯化得到的DNA内毒素水平低于0.1 EU/µg
- 纯化流程快，同时去除内毒素
- 获得高产量、高拷贝质粒DNA
- 使用LyseBlue，获得的裂解和高产量的DNA
QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
质粒纯化方法与转染效率。
使用两种方法独立制备pRSVcat，分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中，及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit（100％）制备的DNA的转染效率表示。
质粒纯化方法与转染效率。
使用两种方法独立制备pRSVcat，分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中，及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit（100％）制备的DNA的转染效率表示。
相关产品订购信息：
| EndoFree Plasmid Maxi Kit (10) | 3890 | 12362 | 10 |
| EndoFree Plasmid Mega Kit (5) | 4720 | 12381 | 5 |
| EndoFree Plasmid Giga Kit (5) | 8080 | 12391 | 5 |
| QIAGEN Large-Construct Kit (10) | 4850 | 12462 | 10 |
| HiSpeed Plasmid Midi Kit (25) | 3840 | 12643 | 25 |
| HiSpeed Plasmid Maxi Kit (10) | 3180 | 12662 | 10 |
| HiSpeed Plasmid Maxi Kit (25) | 7440 | 12663 | 25 |
| CompactPrep Plasmid Midi Kit (25) | 3010 | 12843 | 25 |
| CompactPrep Plasmid Maxi Kit (25) | 6120 | 12863 | 25 |
| QIAGEN Plasmid Plus Midi Sample Kit (5) | 723 | 12941 | |
| QIAGEN Plasmid Plus Midi Kit (25) | 3600 | 12943 | 25 |
| QIAGEN Plasmid Plus Midi Kit (100) | 13240 | 12945 | |
| QIAGEN Plasmid Plus Maxi Sample Kit (5) | 1490 | 12961 | |
| QIAGEN Plasmid Plus Maxi Kit (25) | 7330 | 12963 | 25 |
| QIAGEN Plasmid Plus Maxi Kit (100) | 27180 | 12965 | |
| QIAGEN Plasmid Plus Mega Kit (5) | 3980 | 12981 | 5 |
| QIAGEN Plasmid Plus Giga Kit (5) | 6950 | 12991 | |
| QIAGEN Blood&CellCulture DNA MiniKit(25) | 3390 | 13323 | 25 |
| Blood & Cell Culture DNA Midi Kit (25) | 5400 | 13343 | 25 |
| QIAGEN Blood&CellCulture DNA MaxiKit(10) | 5070 | 13362 | 10 |
| QIAGEN Plasmid Plus 96 Miniprep Kit (4) | 35860 | 16181 | 384 |
| QIArack | 1250 | 19015 | |
| Plasmid Buffer Set | 1410 | 19046 | |
| EndoFree Plasmid Buffer Set | 7430 | 19048 | |
| Buffer P1 (500 ml) | 811 | 19051 | 500 |
| Buffer P2 (500ml) | 811 | 19052 | 500 |
| Buffer P3 (500ml) | 811 | 19053 | 500 |
| Buffer QBT (1000ml) | 1410 | 19054 | 1000 |
| Buffer QC (1000ml) | 1410 | 19055 | 1000 |
| Buffer QF (1000ml) | 1410 | 19056 | 1000 |
| Genomic DNA Buffer Set | 2080 | 19060 | 75 |
| Buffer QG (250 ml) | 666 | 19063 | 250 |
| Buffer N3 (500ml) | 1060 | 19064 | 500 |
| Buffer PE (concentrate, 100ml) | 1060 | 19065 | 100 |
| Buffer PB (500ml) | 1060 | 19066 | 500 |
| Buffer AW/AW2 (concentrate, 324 ml) | 1220 | 19072 | 324 |
| Buffer AVL (155 ml) | 1900 | 19073 | |
| Buffer AL | 1220 | 19075 | 1 |
| Buffer ATL (200ml) | 1220 | 19076 | 200 |
| Buffer AE (240ml) | 1220 | 19077 | 1 |
| Buffer AW1 (concentrate, 242 ml) | 1220 | 19081 | 1 |
| Buffer ASL (4 x 140 ml) | 1370 | 19082 | |
| Buffer PM (500ml) | 837 | 19083 | 500 |
| Buffer EB (250ml) | 423 | 19086 | 250 |
| Reagent DX | 663 | 19088 | 1 |
| Buffer RPW (125ml) | 598 | 19090 | 125 |
| Buffer MTL (54 ml) | 466 | 19112 | 54 |
| QIAGEN Proteinase K (2ml) | 1180 | 19131 | 2 |
| QIAGEN Proteinase K (10 ml) | 4200 | 19133 | 1 |
| QIAGEN Protease (7.5 AU) | 996 | 19155 | |
| QIAGEN Protease (30 AU) | 3200 | 19157 | 30 |
| Collection Tubes (2-ml) | 1830 | 19201 | |
| VacConnectors (500) | 1820 | 19407 | |
| VacValves (24) | 1250 | 19408 | |
| QIAvac 24 Plus | 4300 | 19413 | |
| QIAvac Holder | 1990 | 19418 | |
| QIAvac Connecting System | 13950 | 19419 | |
| QIAvac 96 | 10200 | 19504 | |
| Collection Microtubes (racked, 10 x 96) | 2120 | 19560 | |
| Collection Microtube Caps (120 x 8) | 439 | 19566 | |
| Tape Pads (5) | 822 | 19570 | 5 |
| QIAEX II Gel Extraction Kit (150) | 2340 | 20021 | 150 |
| QIAEX II Gel Extraction Kit (500) | 5350 | 20051 | 500 |
| QIAEX II Suspension (1.5ml) | 1840 | 20902 | 1 |
| Buffer QX1 (500ml) | 1110 | 20912 | 500 |
| R.E.A.L. Prep 96 Plasmid Kit (24) | 54800 | 26173 | 2304 |
| QIAprep Spin Miniprep Kit (50) | 1070 | 27104 | 50 |
| QIAprep Spin Miniprep Kit (250) | 4100 | 27106 | 250 |
| QIAprep 2.0 Spin Columns (100) | 1130 | 27115 | 100 |
| QIAprep 96 Turbo Core Kit (24) | 64690 | 27173 | 2304 |
| QIAprep 96 Turbo Miniprep Kit (4) | 13660 | 27191 | 384 |
| QIAprep 96 Turbo Miniprep Kit (24) | 77320 | 27193 | 2304 |
| QIAprep 96 Plus Miniprep Kit (4) | 15610 | 27291 | 384 |
| DirectPrep 96 Miniprep Kit (4) | 7650 | 27361 | 384 |
| QuickLyse Miniprep Kit (100) | 2420 | 27405 | 100 |
| QuickLyse Miniprep Kit (250) | 5190 | 27406 | 250 |
| MinElute PCR Purification Kit (50) | 1330 | 28004 | 50 |
| MinElute PCR Purification Kit (250) | 6000 | 28006 | 250 |
| MinElute 96 UF PCR Purification Kit (4) | 3710 | 28051 | 384 |
| MinElute 96 UF PCR Purification Kit (24) | 14740 | 28053 | 2304 |
| QIAquick PCR Purification Kit (50) | 1100 | 28104 | 50 |
| QIAquick PCR Purification Kit (250) | 5150 | 28106 | 250 |
| QIAquick 96 PCR Purification Kit (4) | 10180 | 28181 | 384 |
| QIAquick 96 PCR Purification Kit (24) | 40340 | 28183 | 2304 |
| MinElute Reaction Cleanup Kit (50) | 1600 | 28204 | 50 |
| MinElute Reaction Cleanup Kit (250) | 7340 | 28206 | 250 |
| QIAquick Nucleotide Removal Kit (50) | 1410 | 28304 | 50 |
| QIAquick Nucleotide Removal Kit (250) | 6610 | 28306 | 250 |
| MinElute Gel Extraction Kit (50) | 1550 | 28604 | 50 |
| MinElute Gel Extraction Kit (250) | 7120 | 28606 | 250 |
| QIAquick Gel Extraction Kit (50) | 1100 | 28704 | 50 |
| QIAquick Gel Extraction Kit (250) | 5150 | 28706 | 250 |
| Strep-Tactin?Superflow Plus (2 ml) | 2340 | 30002 | 2 |
| Strep-Tactin?Superflow Plus (10 ml) | 9160 | 30004 | 10 |
| Strep-Tactin Superflow Plus Cartrdg(1ml) | 1980 | 30020 | 1 |
| Strep-Tactin Superflow Plus Cartrdg(5ml) | 6370 | 30060 | 5 |
| Ni-NTA Agarose (25 ml) | 3850 | 30210 | 25 |
| Ni-NTA Agarose (100 ml) | 13370 | 30230 | 100 |
| Ni-NTA Agarose (500 ml) | 56230 | 30250 | 500 |
| NTA Agarose (25ml) | 3950 | 30310 | 25 |
| Ni-NTA Superflow (25 ml) | 4740 | 30410 | 25 |
| Ni-NTA Superflow (100 ml) | 15920 | 30430 | 100 |
| Ni-NTA Superflow (500 ml) | 67410 | 30450 | 500 |
| Ni-NTA Fast Start Kit (6) | 4350 | 30600 | 6 |
| Ni-NTA Superflow Columns (12 x 1.5 ml) | 5200 | 30622 | 12 |
| Ni-NTA Superflow Cartridges (5×1 ml) | 2210 | 30721 | 5 |
| Ni-NTA Superflow Cartridges (1×5 ml) | 1960 | 30760 | 1 |
| Ni-NTA Superflow Cartridges (5×5 ml) | 7670 | 30761 | 5 |
| Ni-NTA Superflow Cartridges (100×5 ml) | 122510 | 30765 | 100 |
| QIAamp UCP Pathogen Mini Kit (50) | 4370 | 50214 | 50 |
| QIAamp DNA Blood Mini Kit (50) | 1770 | 51104 | 50 |
| QIAamp DNA Blood Mini Kit (250) | 7530 | 51106 | 250 |
| QIAamp DNA Blood Mini QIAcube Kit (240) | 8450 | 51126 | 240 |
| QIAamp 96 DNA Blood Kit (4) | 14930 | 51161 | 384 |
| QIAamp 96 DNA Blood Kit (12) | 41740 | 51162 | 384 |
| QIAamp DNA Blood Midi Kit (20) | 2590 | 51183 | 20 |
| QIAamp DNA Blood Midi Kit (100) | 11220 | 51185 | 100 |
| QIAamp DNA Blood Maxi Kit (10) | 2590 | 51192 | 10 |
| QIAamp DNA Blood Maxi Kit (50) | 10880 | 51194 | 50 |
| FlexiGene DNA Kit (250) | 3750 | 51206 | 250 |
| FlexiGene DNA AGF3000 Kit (640) | 49630 | 51297 | 640 |
| QIAamp DNA Mini Kit (50) | 2010 | 51304 | 50 |
| QIAamp DNA Mini Kit (250) | 8890 | 51306 | 250 |
| QIAamp DNA Mini QIAcube Kit (240) | 9340 | 51326 | 240 |
| QIAamp 96 DNA QIAcube HT Kit (5) | 8790 | 51331 | 480 |
| QIAamp Fast DNA Tissue Kit, 50 | 1940 | 51404 | 50 |
| QIAamp DNA Stool Mini Kit (50) | 2870 | 51504 | 50 |
| QIAamp Fast DNA Stool Mini Kit (50) | 3190 | 51604 | 50 |
| QIAamp DNA Microbiome Kit (50) | 6620 | 51704 | 50 |
| QIAamp RNA Blood Mini Kit (50) | 4250 | 52304 | 50 |
| QIAamp Viral RNA Mini Kit (50) | 3010 | 52904 | 50 |
| QIAamp Viral RNA Mini Kit (250) | 12980 | 52906 | 250 |
| QIAamp Viral RNA Mini QIAcube Kit (240) | 15660 | 52926 | 240 |
| QIAamp UltraSens Virus Kit (50) | 6030 | 53704 | 50 |
| QIAamp UltraSens Virus Kit (250) | 25230 | 53706 | 250 |
| QIAamp cador Pathogen Mini Kit (50) | 2560 | 54104 | 50 |
| QIAamp cador Pathogen Mini Kit (250) | 12210 | 54106 | 250 |
| cador Pathogen 96 QIAcube HT Kit (5) | 11880 | 54161 | 480 |
| QIAamp Circulating Nucleic Acid Kit (50) | 14160 | 55114 | 50 |
| QIAamp DNA Micro Kit (50) | 2830 | 56304 | 50 |
| QIAamp DNA FFPE Tissue Kit (50) | 2590 | 56404 | 50 |
| QIAamp DNA Investigator Kit (50) | 2990 | 56504 | 50 |
| QIAamp MinElute Media Kit (50) | 4860 | 57414 | 50 |
| QIAamp MinElute Virus Spin Kit (50) | 3180 | 57704 | 50 |
| QIAamp MinElute Virus Vacuum Kit (50) | 4060 | 57714 | 50 |
| QIAamp 96 Virus QIAcube HT Kit (5) | 16960 | 57731 | 480 |
| EpiTect Bisulfite Kit (48) | 2800 | 59104 | 48 |
| EpiTect 96 Bisulfite Kit (2) | 9990 | 59110 | 192 |
| EpiTect Plus DNA Bisulfite Kit (48) | 2250 | 59124 | 48 |
| EpiTect Whole Bisulfitome Kit (25) | 4560 | 59203 | 25 |
| EpiTect MSP Kit (100) | 3600 | 59305 | 100 |
| EpiTect MethyLight PCR Kit (200) | 10340 | 59436 | 200 |
| EpiTect HRM PCR Kit (100) | 1440 | 59445 | 100 |
| EpiTect MethyLight PCR +ROX Kit (200) | 10600 | 59496 | 200 |
| EpiTect Control DNA (1000) | 2730 | 59568 | 1000 |
| EpiTect Control DNA, methylated (100) | 2730 | 59655 | 100 |
| EpiTect Control DNA, unmethylated (100) | 2730 | 59665 | 100 |
| EpiTect PCR Control DNA Set (100) | 5460 | 59695 | 300 |
| EpiTect Fast 96 DNA Bisulfite Kit | 10910 | 59720 | 192 |
| EpiTect Fast 96 FFPE Bisulfite Kit | 19310 | 59740 | 192 |
| EpiTect Fast Bisulfite Kit (10) | 847 | 59802 | 10 |
| EpiTect Fast DNA Bisulfite Kit (50) | 2430 | 59824 | 50 |
| EpiTect Fast DNA Bisulfite Kit (200) | 8290 | 59826 | 200 |
| EpiTect Fast FFPE Bisulfite Kit (50) | 4240 | 59844 | 50 |
| EpiTect Fast LyseAll Bisulfite Kit (50) | 2720 | 59864 | 50 |
| EpiTect Fast LyseAll Bisulfite Kit (200) | 9270 | 59866 | 200 |
| QIAamp DSP Virus Kit (50),CE | 4520 | 60704 | 50 |
| QIAamp DSP DNA Blood Mini Kit (50), CE | 2530 | 61104 | 50 |
| QIAamp DSP Virus Spin Kit (50) | 3580 | 61704 | 50 |
| MagAttract HMW DNA Kit (48) | 3970 | 67563 | 48 |
| DNeasy Plant Maxi Kit (24) | 4590 | 68163 | 24 |
| DNeasy Plant Mini Kit (50) | 2750 | 69104 | 50 |
| DNeasy Plant Mini Kit (250) | 12310 | 69106 | 250 |
| DNeasy 96 Plant Kit (6) | 11590 | 69181 | 576 |
| DNeasy Blood & Tissue Kit (50) | 2140 | 69504 | 50 |
| DNeasy Blood & Tissue Kit (250) | 8810 | 69506 | 250 |
| DNeasy mericon Food Kit (50) | 1990 | 69514 | 50 |
| DNeasy mericon 96 QIAcube HT Kit (5) | 11430 | 69571 | 480 |
| DNeasy 96 Blood & Tissue Kit (4) | 8780 | 69581 | 384 |
| DNeasy 96 Blood & Tissue Kit (12) | 24240 | 69582 | 1152 |
| TissueLyser Single-Bead Dispenser, 5mm | 3520 | 69965 | |
| TissueLyser Single-Bead Dispenser, 7mm | 3520 | 69967 | |
| TissueLyser 3 mm Bead Dispenser, 96-well | 10160 | 69973 | |
| TissueLyser 5 mm Bead Dispenser, 96-well | 10160 | 69975 | |
| TissueLyser LT Adapter, 12-Tube | 17250 | 69980 | |
| TissueLyser Adapter Set (2 x 24) | 16270 | 69982 | |
| TissueLyser Adapter Set (2 x 96) | 12200 | 69984 | |
| RNeasy Protect Animal Blood Kit (50) | 6580 | 73224 | 50 |
| Rneasy Microarray Tissue Mini Kit (50) | 5350 | 73304 | 50 |
| RNeasy Plus Universal Tissue Mini Kit50 | 5330 | 73404 | 50 |
| RNeasy Plus Universal Tissue Midi Kit10 | 2820 | 73442 | 10 |