BD代理APC Mouse IgG2b κ Isotype Control Clone 27-35货号：555745
规格：100T
现货供应
中文名：APC标记Mouse IgG2b κ抗体
BD代理APC Mouse IgG2b κ Isotype Control Clone 27-35货号：555745
Brand
BD Pharmingen™
Alternative Name
Anti-Dansyl
Vol. Per Test
20 µl
Isotype
Mouse C.SW IgG2b, κ
Application
Isotype control, Flow cytometry (Routinely Tested)
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
RUO
BD代理APC Mouse IgG2b κ Isotype Control Clone 27-35货号：555745
产品详情：
INTENDED USE
The mouse IgG2b, κ immunoglobulin isotype control monoclonal antibody 27-35 is specific for the hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing which demonstrated low background staining on a variety of mouse and human tissues.
FORMAT
Format
APC
Excitation Source
Red 633 nm
Excitation Max
650 nm
Emission Max
660 nm
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximay 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Other Formats →
- APC-Cy™7
- APC-H7
- APC-R700
- Alexa Fluor® 488
- Alexa Fluor® 647
- Alexa Fluor® 700
- BB515
- BUV395
- BUV563
- BUV737
- BV421
- BV510
- BV650
- BV711
- BV786
- Biotin
- FITC
- PE
- PE-CF594
- PE-Cy™5
RESOURCES & TOOLS
- Download TDS
STORAGE AND HANDLING
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.