葡聚糖凝胶G-50Sephadex G-50

CAS号：9048-71-9

英文名称：Sephadex G-50
其他名称：交联葡聚糖凝胶G-50
CAS号：9048-71-9
干颗粒直径：40～120um
分子量分级范围：A.肽及球形蛋白质：1500～30000；B. 葡聚糖（线性分子）：500～10000
床体积毫升/克干分子筛：9～11ml/g
得水值：5.0±0.3
溶胀zui少平衡时间（h）：室温6h（20℃）；沸水浴2h（90℃）
PH：2～13
性状：白色珠粒，属亲水性凝胶。性稳定，不溶于水、弱酸和碱溶液，遇强酸能使糖苷键分解

葡聚糖凝胶G-50Sephadex G-50

Sephadex G-50 Medium, 100 g

Sephadex G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
• Suitable for purification of DNA from small molecules by gel filtration
• Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
• Classic gel filtration medium.

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

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