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Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.
Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.
This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.
Download in ChemDraw format
Cy5.5 absorbance and emission spectra
Recommended protocol
NHS ester labeling of amino biomolecules
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Protein labeling calculator
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Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.