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Water soluble Cyanine 5 succinimidyl ester (SE), an equivalent of Cy5® NHS ester, for the labeling of various amine-containing molecules in aqueous phase without use of any organic co-solvent. This product is therefore particularly useful for the labeling of proteins which denature in the presence of organic co-solvents, as well as for proteins with low solubility.
Sulfo-Cyanine 5 is an analog of Cy5®, one of the most popular fluorophores which is compatible with various equipment such as plate readers, microscopes, and imagers.
This dye is highly hydrophilic and water-soluble. A non-sulfonated analog is also available.
Can be used as a replacement for Cy5®, Alexa Fluor 647, DyLight 649 for all applications.
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Sulfo-Cyanine 5 absorbance and emission spectra
Recommended protocol
NHS ester labeling of amino biomolecules
Calculator
Protein labeling calculator
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Sulfo-Cyanine5 alkyne
Sulfo-Cyanine5 is a water-soluble Cyanine5 dye alkyne for Click chemistry.