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Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.
Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.
For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.
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Cy5 excitation and emission spectra
Recommended protocol
Maleimide labeling of proteins and other thiolated biomolecules
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Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper free Click chemistry.
