产品货号：ZZK-K-LMALR

产品品名：L-苹果酸检测试剂盒

英文品名：L-Malic Acid (Regular) Assay Kit

规格型号：58 assays (manual) / 580 assays (microplate)

For the specific assay of L-malic acid (L-malate) in beverages and food products.

INTRODUCTION:

As a component of the citric acid cycle, L-malic acid (L-malate)

is found in all living organisms. Its quantitative determination is

especially important in the manufacture of wine, beer, bread, fruit

and vegetable products, as well as in cosmetics and pharmaceuticals.

It is one of the most important fruit acids, and has the highest

concentration of all acids in wine. In the wine industry, the level of

L-malic acid is monitored, along with L-lactic acid, during malolactic

fermentation. L-Malic acid finds many applications as a food

preservative (E296) and flavour enhancing compound, such as in the

manufacture of low calorie drinks.

This booklet describes a manual spectrophotometric and a microplate

method for the quantitative determination of L-malic acid. However,

an alternative method tailored towards the small winery is also

available from Megazyme that is based on the use of an inexpensive

colorimeter, the MegaQuantTM meter (see product D-LMALMQ).

A third kit for L-malic acid has been optimised for auto-analyser

applications (see product K-LMALAF).

KITS:

Kits suitable for performing 58 / 116 assays in manual format (or 580 /

1160 assays in microplate format) are available from Megazyme. The kits

contain the full assay method plus:

58 Determinations Kit (cat. no. K-LMALR)

Bottle 1: Buffer (6 mL, pH 10.0) plus L-Gluta mate and sodium

azide (0.02% w/v) as a preservative.

Stable for 大于2 years at 4°C.

Bottle 2: NAD+ plus PVP.

Stable for 大于5 years at -20°C.

Bottle 3: Gluta mate-oxaloacetate transaminase suspension

(1.25 mL).

Stable for 大于2 years at 4°C.

Bottle 4: L-Malate dehydrogenase suspension

(1.25 mL).

Stable for 大于2 years at 4°C.

Bottle 5: L-Malic acid standard solution (5 mL, 0.15 mg/mL).

Stable for 大于2 years at 4°C.

116 Determinations Kit (cat. no. K-LMALL)

Bottle 1: Buffer (12 mL, pH 10.0) plus L-Gluta mate and sodium

azide (0.02% w/v) as a preservative.

Stable for 大于2 years at 4°C.

Bottle 2: (x2) NAD+ plus PVP.

Stable for 大于5 years at -20°C.

Bottle 3: Gluta mate-oxaloacetate transaminase suspension

(2.5 mL).

Stable for 大于2 years at 4°C.

Bottle 4: L-Malate dehydrogenase suspension (2.5 mL).

Stable for 大于2 years at 4°C.

Bottle 5: L-Malic acid standard solution (5 mL, 0.15 mg/mL).

Stable for 大于2 years at 4°C.

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