产品货号：ZZK-K-DMAL

产品品名：D-苹果酸检测试剂盒

英文品名：D-Malic Acid Assay Kit

规格型号：100 assays (manual) / 1000 assays (microplate) / 1100 assays(auto-analyser) per kit

For the specific assay of D-malic acid (D-malate) in beverages and food products.

INTRODUCTION:

In Nature, essentially all malic acid is produced in the L-racemic

form (the exception being a few micro-organisms which produce

D-malic acid). Freshly pressed fruit juices contain D-malic acid at

the detection limit (~ 10 mg/L). Malic acid is produced commercially

in the D-/L-racemic mixture by the catalytic hydration of maleic

anhydride and can replace citric acid in food products as an acidulant

and flavour enhancer. It finds application in flavoured fruit drinks,

juices and wines. The detection of D-malic acid in juices or wine

indicates that it has been added. The legal situation concerning the

addition of D-/L-malic acid to juice or wine varies between countries.

KITS:

Kits suitable for performing 100 assays in manual format (or 1100 assays

in auto-analyser format or 1000 assays in microplate format) are available

from Megazyme. The kits contain the full assay method plus:

Bottle 1: Buffer (25 mL, pH 8.0) plus sodium azide (0.02% w/v)

as a preservative.

Stable for > 2 years at 4°C.

Bottle 2: (x2) NAD+.

Stable for > 5 years at -20°C.

Bottle 3: D-Malate dehydrogenase suspension (2.2 mL).

Stable for > 2 years at 4°C.

Bottle 4: D-Malic acid standard solution (5 mL, 0.20 mg/mL).

Stable for > 2 years at room temperature.

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