产品货号：ZZK-K-ASPTM 

产品品名：阿斯巴甜检测试剂盒

英文品名：Aspartame Assay Kit

规格型号： 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)

A simple and reliable method for the specific determination ofaspartame in beverages and foodstuffs.

INTRODUCTION:

Aspartame is a dipeptide sweetener comprising L-aspartate and

methyl esterified L-phenylalanine (Asp-Phe-O-Me). At 200 times the

sweetness of sucrose, and with a structure similar to dipeptides found

in a normal diet, its use in low calorie carbonated drinks and other

foodstuffs has been widely accepted since the early 1980s. However,

due to its L-phenylalanine content, consumption of this compound

must be strictly monitored by those suffering from phenylketonuria.

There is also a significant level of concern voiced by some who

believe this sweetener poses serious health problems relating to the

three components, L-aspartic acid, L-phenylalanine and methanol,

though there is, as yet, no evidence to support such claims.

KITS:

Kits suitable for performing 50 assays in manual format (or 500

assays in auto-analyser format or 500 assays in microplate format) are

available from Megazyme. The kits contain the full assay method plus:

Bottle 1: Buffer (12 mL, pH 8.0) plus 2-oxoglutarate and

sodium azide (0.02% w/v) as a preservative.

Stable for > 2 years at 4°C.

Bottle 2: (x2) NADH plus stabiliser.

Stable for > 5 years at -20°C.

Bottle 3: L-Malate dehydrogenase plus glutamate-oxaloacetate

transaminase suspension (1.1 mL).

Stable for > 2 years at 4°C.

Bottle 4: Peptidase M suspension (1.1 mL).

Stable for > 2 years at 4°C.

Bottle 5: Aspartame control powder (~ 1 g, ~ 2.5% w/w

aspartame on an “as is” basis. The exact

concentration of aspartame is given on the vial

label).

Stable for > 5 years when stored dry at 4°C.

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