产品货号：ZZK-K-LACTUL

产品品名：乳果糖检测试剂盒

英文品名：Lactulose Assay Kit

规格型号：50 assays

For the specific, rapid and sensitive measurement of Lac tulose in milkand milk-based samples. Reagents included in this kit may also beprepared for use in the procedure described by ISO Method 11285:2004.

INTRODUCTION:

Thermal treatment of milk is an essential process in the dairy

industry. Various heating regimens can be employed, and thus in

some cases it becomes necessary to determine thermal history

accurately. This can be achieved by quantitative measurement of

Lac tulose, a disaccharide comprising D-galactose and D-fructose

that is only produced from the common milk sugar lactose at

elevated temperatures. In raw milk the level of Lac tulose is very low.

However, as heat treatment increases this sugar can eventually reach

~ 2 g/L (in the case of UHT milk for example).

This kit (K-LACTUL) is suitable for the measurement of Lac tulose

in milk-based products such as fresh milk, UHT milk, evaporated

milk and powdered milk. However, this kit is unsuitable for the

measurement of Lac tulose in samples that have been artificially

sweetened, such as condensed milk, where the “free fructose” to

Lac tulose ratio is extremely high.

Reagents included in this kit can be prepared for use in the

measurement of Lac tulose using the procedure as described by

ISO Method 11285:2004. However, the slightly modified advanced

format K-LACTUL (Rapid Method) developed by Megazyme, that

is both more sensitive and rapid, features an additional enzyme,

6-phosphogluconate dehydrogenase, which results in the sensitivity of

the method being improved 2-fold. Additionally, optimised selection

and use of highly purified enzymes results in a method that can easily

be performed within one working day.

KITS:

Kits suitable for performing 50 assays are available from Megazyme.

The kits contain the full assay method plus:

Bottle 1: Beta-Galactosidase suspension (3 mL).

Stable for 大于 2 years at 4°C.

Bottle 2: Glu-cose oxidase and catalase, lyophilised powder.

Stable for 大于 2 years at -20°C.

Bottle 3: Buffer (6 mL, pH 7.6) plus sodium azide (0.02% w/v)

as a preservative.

Stable for 大于 2 years at 4°C.

Bottle 4: NADP+ plus ATP.

Stable for 大于 2 years at 4°C.

Bottle 5: Hexokinase plus Glu-cose-6-phosphate dehydrogenase

suspension, 2.2 mL.

Stable for 大于 2 years at 4°C.

Bottle 6: 6-Phosphogluconate dehydrogenase suspension

(2.2 mL).

Stable for 大于 2 years at 4°C.

Bottle 7: PhosphoGlu-cose isomerase suspension (2.2 mL).

Stable for 大于 2 years at 4°C.

Bottle 8: Lac tulose (0.1 mg/mL) plus D-fructose (0.05 mg/mL)

standard solution (5 mL).

Stable for 大于 2 years at 4°C.

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